转 ATCIPK23基因烟草植株钾素营养效率研究OA北大核心CSCDCSTPCD

The experiment was conducted to study the ability of K+ and dry matter accumulation of ATCIPK23 transgenic tobacco lines under different K+ concentration .The results showed that there were remarkable growth advan-tages for the transgenic tobacco lines ,whose plant growth rate ,root development ,dry matter accumulation and plant potassium content were wholly improved remarkably ,compared with the contrast variety .These effects tended to become stronger along with the reduction of potassium supply .When the K+ concentration of culture medium dropped to 0 .1 mmol・L-1 ,the potassium content in the above-ground part of contrast variety K326 was 17 .7 g・kg-1 ,and that of the transgenic lines KA12 ,KA16 and KA21 was 23 .3 g・kg-1 ,24 .6 g・kg-1 and 22 .1 g・kg-1 respectively ,being in-creased by 31 .6% ,39 .0% and 27 .7% respectively .The study on potassium absorption kinetics indicated that there was no significant difference in maximum absorption efficiency (Vmax ) between the transgenic lines and the contrast vari-ety ,but there were significant differences in minimum concentration (Cmin ) and Michaelis constant (KM ) between them .