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https://opaj.napstic.cn/periodicalArticle/downloadReview/0120131000107796
胃泌素对大鼠小肠上皮1,25(OH)2D3膜相关快速反应类固醇结合蛋白的影响OA北大核心CSCDCSTPCD
Effects of gastrin on rat intestinal epithelial 1,25(OH)2D3-membrane associated rapid response steroid binding protein
目的 探讨胃泌素对大鼠小肠上皮1,25(OH)2D3膜相关陕速反应类固醇结合蛋白(1,25D3-MARRS结合蛋白)表达的影响.方法 应用免疫组织化学、RT-PCR和免疫印迹法,检测胃泌素、奥美拉唑处理后的SD大鼠小肠中1,25D3-MARRS结合蛋白的表达情况与对照组的差异,并测大鼠血清中钙、磷离子的浓度变化.结果 大鼠小肠上皮细胞中1,25D3-MARRS结合蛋白分布于胞核、胞浆和胞膜.胃泌素、奥美拉唑均引起大鼠小肠组织1,25D3-MARRS结合蛋白的mRNA和蛋白两个水平表达增高(P＜0.05),但其作为一个促小肠钙磷吸收的蛋白,无明显增高血清钙磷浓度的作用(P＞0.05).结论 1,25D3-MARRS结合蛋白作为一个广泛分布的多功能蛋白,受胃泌素的调节,在消化道相关疾病具有很高的研究价值及临床应用前景.
Objective To explore the effects of gastrin on the expression of 1,25(OH)2D3-membrane associated rapid response steroid (1,25D3-MARRS) binding protein in rat intestinal epithelium.Methods SD rats received intraperitoneal injections of gastrin,omeprazole or physiological saline.The protein expression of 1,25D3-MARRS binding protein in SD rat intestinal was determined with Western blotting and immunohistochemistry,and its mRNA levels determined by RT-PCR.The serum calcium and phosphate levels in the rats were also detected.Results Immunohistochemistry showed that 1,25D3-MARRS binding protein was expressed mainly in the nuclei,cytoplasm and membrane of the intestinal epithelial cells.Both the protein and mRNA expression levels of 1,25D3-MARRS binding protein were up-regulated after treatments with gastrin and omeprazole (P＜0.05),but the serum calcium and phosphate concentrations showed no obvious increase.Conclusion 1,25D3-MARRS binding protein,which is widely expressed with versatile functionalities,is regulated by gastrin and shows high potentials in the study of gastrointestinal diseases.
梁芬芬;刘翠平;黎丽旋;郭瑜;白岚
南方医科大学南方医院消化内科//省胃肠疾病重点实验室,广东广州510515南方医科大学南方医院消化内科//省胃肠疾病重点实验室,广东广州510515南方医科大学南方医院消化内科//省胃肠疾病重点实验室,广东广州510515南方医科大学南方医院惠侨科,广东广州510515南方医科大学南方医院惠侨科,广东广州510515
1,25D3-MARRS结合蛋白胃泌素小肠钙磷吸收
1,25D3-MARRS binding proteins gastrin calcium and phosphate uptake intestines
《南方医科大学学报》 2013 (7)
甲状旁腺激素相关蛋白促进小肠上皮细胞钙吸收的分子机制
990-993,4
国家自然科学基金(81170354)广东省科技计划项目(2011B031800195)Supported by National Natural Science Foundation of China (81170354).
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