大豆组培苗水培生根与培养基生根比较研究OA北大核心CSCDCSTPCD

Root-inducing is the final step for producing regenerated plantlets in the tissue culture of soybean [Glycine max (L.) Merr.].Here an easy,efficient root-inducing system for soybean shoots is reported,which is designated as tap-water rooting system,in which tap water is (without autoclaving)used to replace the traditional agar-containing medium for root induction and growth.Taking Zigongdongdou as material,the results demonstrated that the tap-water rooting system was good for shortening culture time,overcoming contamination of bacterium and fungus,increasing the rooting efficiency and decreasing the cost of tissue culture as exemplified by faster root inducing and elongating,and higher plantlet survive ratio of the tap-water system than that of agar-containing medium system.Although the amount of total roots from the tap-water system was less than that from agar plates,the plantlets from tap-water system could grow,flower and produce pods and seeds normally and no significant difference were found between the tap-water system and agar system.Hence,it is suggested that the easy rooting system should be used in the tissue culture of more soybean cultivar.