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25羟维生素D3对支气管上皮细胞维生素D受体表达及分布的影响OA北大核心CSCDCSTPCD
Effect of 25-hydroxyvitamin D3 on vitamin D receptor expression and distribution in human bronchial epithelial cells in vitro
目的 探讨25羟维生素D3对人正常气道上皮维生素D受体表达及分布的影响.方法 选取正常人支气管上皮细胞系16HBE为研究对象,MTT法分别检测不同浓度25羟维生素D3对细胞活力的影响；实验组分为对照组和不同浓度25羟维生素D3处理组.各处理因素作用细胞时间为24 h.用实时荧光定量PCR、Western blot、免疫荧光法观测各处理组维生素D受体(VDR)mRNA及蛋白表达、分布的情况.结果 与对照组相比,各个不同处理浓度的25羟维生素D3对VDR的表达量及分布均无显著增加(P＞0.05).结论 25羟维生素D3对支气管上皮细胞的影响可能并不依赖于VDR的表达及转位.
Objective To evaluate the effect of 25-hydroxyvitamin D5 on the expression and distribution of vitamin D receptor in normal human bronchial epithelial cells. Methods MTT assay was used to assess the "viability of human airway epithelial cell line 16HBE following a 24-h exposure to different concentrations of 25-hydroxyvitamin D3. Real-time quantitative PCR, Western blotting, and immunofluorescence assay were used to observe the expression and distribution of vitamin D receptor in the cells following the exposure. Results Compared with the control cells, 16HBE cells exposed to different concentrations of 25-hydroxyvitamin D3 exhibited no significantly increase in the expression or distribution of vitamin D receptor. Conclusion The influence of 25-hydroxyvitamin D3 on bronchial epithelial cells might be independent of the expression and translocation of vitamin D receptor.
董航明;赵海金;刘来昱;梁振宇;吕燕华;蔡绍曦
南方医科大学南方医院呼吸科,广东广州510515南方医科大学南方医院呼吸科,广东广州510515南方医科大学南方医院呼吸科,广东广州510515南方医科大学南方医院呼吸科,广东广州510515南方医科大学南方医院呼吸科,广东广州510515南方医科大学南方医院呼吸科,广东广州510515
医药卫生
人气道上皮细胞25羟维生素D3维生素D受体
human airway epithelium 25-hydroxy vitamin D3 vitamin D receptor
《南方医科大学学报》 2012 (1)
维生素D相关蛋白VDUP1对气道上皮细胞调控哮喘炎症关键分子TSLP的影响
28-31,4
国家自然科学基金(30971328)教育部博士点基金(20094433110011)南方医院院长基金(20102002)
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